ASTM E3238-20,Standard Test Method for Quantitative Measurement of the Chemoattractant Capacity of a Nanoparticulate Material in vitro.
ASTM E3238-20 provides a protocol for rapid and quantitative measurement of the chemoattractant capacity of a nanoparticulate material (nanoparticles and their aggregates and agglomerates).
This test method describes a protocol for assessing and measuring the chemoattractant capacity of nanoparticulate material.
The migration of cells through a filter towards the nanoparticulate material is monitored and quantified after staining with calcein AM dye.
Calcein AM, a non-fluorescent hydrophobic compound,is transported through the cell membrane into live cells.Intracellular esterases remove the acetomethoxy group of calcein AM producing calcein, a hydrophilic green fluorescent compound. Calcein is retained by the live cells.