BS EN 1390:2020 pdf download.Wood preservatives – Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae – Laboratory method.
For fast and slow acting wood preservatives, allocate three larvae from the 51 mg to 100 mg weight range and three larvae from the 101 mg to 150 mg mass range to each test specimen. Carefully insert the larvae (5.1) head first into the appropriately sized holes.
For deferred acting wood preservatives, allocate one larva from the 51 mg to 100 mg weight range and two larvae from the 101 mg to 150 mg weight range to each test specimen (or vice versa). Carefully insert the larvae (5.1) head first into the appropriately sized holes. Insert one larva from one mass range into the middle hole at one end of the test specimen and two larvae from the other mass range into outer holes of the other end of the test specimen.
Seal the insertion holes with plugs of cotton wool. Incubate the test specimens for one week in the testing chamber (5.3.3), then remove the cotton wool plugs and determine whether each larva has bored, replacing larvae, which have not bored. If any larvae are replaced then incubate all test specimens for a further week in the testing chamber (5.3.3).
8.3 Sealing of the surfaces not to be treated
Seal the 100mm x 150 mm pith face and the two cross sections.
a) For tests with preservative solutions in which water is the continuous phase, apply three coats of paraffin wax (5.2.1) at about 90 °C so that the first coat adheres closely to the wood and the successive coatings bond to one another;
b) for tests with preservative solutions in which the continuous phase is an organic solvent that dissolves paraffin wax, use the gelatine (5.2.2). Apply the first coat with an aqueous solution (200 g/l) at about 40°C, then after a minimum of 8 h of drying. apply two further coats of an aqueous solution (300 g/l) at about 50 °C;
c) other types of coating may be used if they are non-toxic to the test insects (5.1.1) and if their performance as sealants has been documented.
8.4 Incubation of the test specimens
Incubate all the sealed test specimens in the testing chamber (5.3.3) for a maximum of two weeks from the date at which larvae were first inserted.
8.5 Treatment of test specimens
8.5.1 Preparation of the treatment solution
8.5.1.1 Solid wood preservatives
Water-soluble wood preservatives:
dissolve the wood preservative in the water (5.2.3) to the required working concentration.
Non-water-soluble wood preservatives:
dissolve the wood preservative in an appropriate solvent (5.2.4) to the required working concentration.
8.5.1.2 Liquid wood preservatives
If appropriate, use the wood preservative without further preparation other than any necessary stirring. If it is a concentrate, dilute the wood preservative with the diluent to the required working concentration, using the procedure specified by the manufacturer. For the dilution of water-based wood preservatives use the water specified in 5.2.3.
All treatment solutions shall be freshly prepared.
8.5.2 ApplIcation of the treatment solution
Remove the test specimens to be treated from the testing chamber (5.3.3) after completion of the incubation period (8,4). Determine the actual area of each unsealed surface to be treated taking into account any possible encroachment of the sealing compound.
NOTE The total area to be treated is theoretically 225 cm2.
Determine the volumes or masses of the treatment solution (8.5.1) to be applied to each unsealed face to give the application rate specified by the supplier.
The quantity of the treatment solution to be applied should be realistic in view of the field of application and the manufacturer’s instructions. In the fume cupboard (5.3.2), using either the pipette (5.3.5) or a brush (5.3.6), apply the appropriate calculated volume or mass of the treatment solution (8.5.1) to each of the unsealed faces as uniformly as possible. Apply the treatment solution to each unsealed face whilst keeping that face in a horizontal and upward facing position. Allow any surface liquid to be absorbed into each face before treating the next face.
II the required quantity cannot be applied in one application, the treatment solution may he applied in successive applications at appropriately close intervals so as to avoid solidification of any substances hindering the penetration of the subsequent applications.
If a brush application is used, weigh the test specimens during application to determine the mass applied.
From the quantity of the treatment solution applied to each unsealed face of each treated test specimen, determine and record the average application rate in g/m2 (brush application), or mI/rn2 (pipette application) of the treated test specimens.
Complete the treatment of all faces of each set of test specimens within 3 h.BS EN 1390 pdf download.