EN 46-2-2016 pdf download.Wood preservatives – Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) – Part 2: Ovicidal effect (laboratory method).
If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a concentrate, dilute the preservative with the diluent specified by the manufacturer to the required working concentration.
A formulation of the preservative to be tested without active ingredients shall also be included in the test.
All treatment solutions shall be freshly prepared.
8.1.3.2 Treatment by brushing or by pipette
In the laboratory work area (5.3.3) apply the preservative either by brushing or by pipette to the 50 mm x 25 mm lateral face that would have been furthest from the centre of the tree.
Depending on the type of treatment, the volume (application by pipette) or mass (application by brushing) of the treatment solution shall be determined to obtain the surface application specified by the manufacturer. Place the specimens such that the 50 mm x 25 mm lateral face that is to be treated is uppermost and apply the appropriate fluid uniformly to that face.
When the preservative is applied by brush, place the test specimens on a balance while being brushed to determine the amount of preservative applied to the nearest 0,01 g.
When the preservative is applied by pipette, move the pipette across the fibre direction and the amount of preservative applied shall be determined to the nearest 0.01 ml.
Several applications can be necessary to apply the required amount. In this case the coats should be applied sufficiently quickly to avoid any solidification of certain substances which can impede the penetration of further coats.
Care shall be taken to avoid fluid running off the lateral face being treated.
8.1.3.3 Treatment by dipping
When the treatment of specimens is by dipping all faces, except one 25 mm x 50 mm face, shall be sealed (see 8.1.2).
Weigh to the nearest 0,01 g any sealed test specimen, to obtain its initial mass.
Immerse one test specimen after the other in the treatment solution, moving it during dipping. The dipping time to be used shall be one of the following, agreed beforehand according to the purpose of the test:
— Either one lOs period and/or two periods of lOs at an interval of 24 h.
If the rate of solidification of some constituents of a preservative formulation would have the effect of retarding its penetration during the second dipping, this interval has to be reduced. The interval employed shall be mentioned In the test report.
Using forceps, remove each test specimen from the preservative fluid and sponge off fluid from all the sealed faces of the specimen. Keeping the face that has not been sealed upper most, immediately weigh to the nearest 0.01 g.
In the case of water-soluble chemicals, for example salts, and water-insoluble chemicals which are being studied as active ingredients, calculate the mass of chemical retained for each test specimen from the mass of solution absorbed and its concentration.
In the case of organic formulations or organic water-dispersible formulations, the retention is expressed for each test specimen in terms of the corresponding mass of the formulation ready for use, but if a concentrate is supplied the retention is expressed in terms of the dilution applied.
Calculate the mass of preservative retained in grams per square meter of timber surface.
8.1.4 Drying and conditioning of the test specimens after treatment
If the end-sealing has been damaged before or after treatment, reject the test specimens concerned from the tests.
After treatment, condition the test specimens for four weeks in the environment specified for the conditioning chamber (5.3.2). Arrange the test specimens on their lateral narrow faces, resting on glass rods, not touching one another. Invert the test specimens twice a week.
NOTE The drying and conditioning of the test specimens depend on the nature of the product under test and on the solvent or diluent used. For slow drying products It may be necessary to extend the conditioning process.
If, in the case of slow drying products, the conditioning period is extended, the extended conditioning period shall be stated in the test report.
If the test specimens are to be subject to an ageing procedure (according to EN 73 or EN 84), this shall be carried out after this drying procedure.
8.2 Exposure of the test specimens to the insects (ovicidal action)
Mate young Hyiotrupes females in Petri dishes (5.3.5). After mating transfer the females singly to other Petri dishes (5.3.5) with centrally placed test specimens (7.5,1), Place the treated face of the test specimen on the bottom of the Petri dish leaving a gap for egg-laying. The test specimens are now ready for egg-laying by the house longhorn beetle.
Egg-laying extends over seven days under the conditions of the testing chamber (5.3.4). After the first egg-laying remove the females.EN 46-2-2016 pdf download.