EN 49-1-2016 pdf download.Wood preservatives – Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival – Part 1: Application by surface treatment (Laboratory method).
In the case of organic formulations or organic water-dispersible formulations, the retention is expressed for each test specimen in terms of the corresponding mass of the formulation ready for use, but if a concentrate is supplied the retention is expressed in terms of the solution prepared ready for use as specified by the manufacturer.
Calculate the mass of preservative retained per unit area of unsealed wood surface.
8.1.3.2.2 Treatment by pipette application
Determine the actual area of the unsealed face to be treated taking into account any possible encroachment of the sealing compound.
NOTE 1 The area to be treated is theoretically 12,5 cmZ.
Determine the volumes or masses of the treatment solution (8.1.3.1) to be applied to the unsealed face to give the application rate specified by the supplier.
The quantity of treatment solution to be applied should be realistic in view of the field of application and the manufacturers instructions. Normally the quantity should not exceed 100 g/m2.
In the laboratory work area (5.3.4), using the pipette (5.3.6) apply the calculated volume or mass of the treatment solution (8.1.3.1) to the unsealed faces as uniformly as possible. Apply the treatment solution to each unsealed face while keeping that face in a horizontal and upward facing position. Allow any surface liquid to be absorbed, make a mark to indicate this face for further operations.
NOTE 2 If the required quantity cannot be applied in one application, the treatment solution may be applied in successive applications at appropriately close intervals so as to avoid solidification of any substances hindering the penetration of the subsequent applications.
From the quantity of treatment solution applied to the unsealed face of each treated test specimen, determine and record the application rate in grams per square metre or millilitres per square metre of the treated test specimens.
Treat the control test specimens (7.5 c)) in an identical manner using solvent or diluent (5.2.4 or 5.2.5) if solvent or diluent are used in the preparation of the treatment solution.
8.1.4 Drying and conditioning of the test specimens after treatment
If the sealing has been damaged before or after treatment, reject the test specimens concerned from the tests.
After treatment, condition the test specimens for four weeks in the environment specified for the conditioning chamber (5.3.2). Arrange the test specimens on their narrow faces, resting on glass rods, not touching one another. Invert the test specimens twice a week.
8.2 Exposure of the test specimens to the insects
Prepare the egg-laying zones by attaching a piece of the fine cloth (5.2.7) measuring approximately 45 mm x 20 mm to the unsealed face of the test specimen. Use the paste (5.2.3) to attach the cloth and smooth this out so that the mesh openings are not twisted.
immediately prior to exposure to egg-laying, condition all the test specimens for one week in the testing chamber (5.3.5).
Place each test specimen in one of the test containers (5.3.8) and add five female insects and at least five male insects. Cover the container with a disc of filter paper (5.2.6). Keep this in place with the cover.
8.3 Conditions and duration of the test
Place the containers containing the test specimens and the insects in the testing chamber (5.3.5) for approximately one week Count the eggs on each test specimen and, If there are fewer than 50, add another group of insects to the container and count the eggs again at the end of a further week in the testing chamber
(5.3.5).
Each control test specimen should have at least 50 eggs for the test to be valid.
NOTE It may be necessary to add further insects in order to obtain an adequate number of eggs on all the test specimens. However, premature mortality of the insects on the treatment test specimens alone may be due to the action of the preservative.
When premature mortality of the insects occurs, this shall be mentioned in the test report. II 50 eggs have not been laid on treated test specimens after four groups of five pairs of insects have been added, continue without adding further insects and note this in the test report. When, at the end of several weeks, all the insects are dead, they shall be removed and the test specimens left in the containers in the testing chamber (5.3.5). Examine the test specimens 26 weeks or 52 weeks after introducing the last insects, depending on the expected mode of action of the test product.
8.4 Examination of the test specimens
52 weeks after introducing the last insects (26 weeks respectively), count as accurately as possible the number of eggs laid on each test specimen and the number of eggs that have hatched). Cut up all the test specimens and count the larvae, noting their general condition.
NOTE Evaluation of the presence and size of larvae in the test specimens may be carried out at intervals during the test using the X-ray apparatus (5.3.10), if available.EN 49-1-2016 pdf download.