EN 15510-2017 pdf download.Animal feeding stuffs: Methods of sampling and analysis -Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum and lead by ICP-AES.
5.9 Element stock solutions
Ca, Na. P. Mg, K, Fe, Zn, Cu. Mn, Co. Mo, Pb
c = 1 000 mg/I.
The user should choose a suitable stock solution. Both single-element stock solutions and multi-element stock solutions with adequate specification stating the acid used and the preparation technique are commercially available. It is advisable to use certified stock solutions.
Stock solutions are not to be used after the expiry date. Element stock solutions with concentrations different from 1 000 mg/I may also be used.
5.10 Standard solutions
5.10.1 General
Depending on the scope, dittérent multi-element standard solutions may he necessary. Jn general, when combining multi-element standard solutions, their chemical compatibility and the possible hydrolysis of the components shall be regarded. Spectral interferences from other elements in multi-element standard solutions also need to be considered (Annex B, B.2.2). The examples given below also consider the measuring range of various inductively coupled plasma atomic emission spectrometers and the expected concentration of the element in animal feeding stuffs.
The multi-element standard solutions are considered to be stable for several months, if stored in the dark.
Other combinations of elements at different concentrations can be used, provided that the element stock solutions (5.9) are diluted with the same acid and equal concentration as the acid in the test solution to a range of standards that covers the concentrations of the elements to be determined.
5.10.2 Multi-element standard solution — Ca, Na, P, Mg, K in I % HCI
c (Ca, Na, P. Mg, K) = 40 mg/I
Pipette 40,0 ml of each element stock solution (Ca, Na, P, Mg, K) (5.9) into a 1 000 ml volumetric flask (6.7). Add 60 ml of dilute hydrochloric acid (5.7). Fill to the mark with water and transfer to a suitable storage bottle.
5.10.3 Multi-element standard solution – Fe, Zn, Cu, Mn, Co, Mo in 1 % HCI
c (Fe, Zn, Cu, Mn, Co, Mo) = 50 mg/I
Pipette 50,0 ml of each element stock solution (Fe, Zn, Cu, Mn. Co, Mo) (5.9) into a 1 000 ml volumetric flask (6.7). Add 60 ml of dilute hydrochloric acid (5.7). Fill to the mark with water and transfer to a suitable storage bottle.
6.1.2 Laboratory grinder capable of grinding to a particle size of less than or equal to 1 mm, e.g. a knife mill or equivalent.
6.1.3 Laboratory grinder for mineral matrices capable of grinding to a particle size of less than or equal to 0,5 mm, e.g. a ball mill, centrifuge mill or equivalent.
6.1.4 Mortar with pestle, free of contamination.
6.2 Analytical balance, capable of weighing to an accuracy of 1 mg.
6.3 Electric hot plate, with temperature control.
6.4 Ashing crucibles, of platinum, quartz or porcelain.
6.5 Electric muffle-furnace, capable of being maintained ata temperature of 450°C ± 20°C.
The real temperature in the furnace has to be checked, because this temperature may be substantially different from the adjust temperature.
6.6 Beaker, of capacity 250 ml.
6.7 One-mark volumetric flasks, of capacities 100 ml, 500 ml, 1 000 ml.
6.8 Inductively coupled plasma — Atomic Emission Spectrometer
The instrument shall be equipped with a radial plasma as a minimum requirement; an axial plasma is equally acceptable. Background correction shall also be performed when necessary. Settings of the working conditions (e.g. viewing height, gas flows, RF or plasma power, sample uptake rate, Integration time, number of measurements) shall be optimized according the manufacturer’s instructions.
6.9 Freeze drying equipment, capable of freeze-drying liquid animal feeding stuffs.
6.10 Oven for pre-drying, capable to hold a temperature of (70±5) °C.
7 Sampling
Sampling is not part of the method specified in this Standard. A recommended sampling method is given in EN ISO 6497 [2].
It is important that the laboratory receives a sample that is truly representative and has not been damaged or changed during transport or storage.EN 15510-2017 pdf download.