BS ISO 21160:2018,Cigarettes — Determination of selected carbonyls in the mainstream smoke of cigarettes — Method using high performance liquid chromatography.
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BS ISO 21160:2018 includes recommendations about some of the critical steps that should be controlled to provide data as robust and consistent as the repeatability and reproducibility data provided in the ISO document. Statistical evaluations were carried out according to lSO 5725-1 and ISO 5725-2.
BS ISO 21160:2018 is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette using reversed phase HPLC-UV/DAD.
Cigarettes are smoked on a standard smoking machine as specified in ISO 3308 that has been fitted with impingers, but without a glass fibre filter pad as described in ISO 3308 and the filter pad holder, under the ISO 3308 smoking regime.
The carbonyls in mainstream tobacco smoke are trapped by passing each puff through an impinger device containing an acidified solution of 2, 4-dinitrophenylhydrazine (DNPH) in 1: 1 acetonitrile:water:
An aliquot of the smoke extract is then syringe-filtered and diluted with 1%  tris-(hydroxymethyl)-aminomethane in aqueous acetonitrile.
BS ISO 21160:2018 pdf is free to download.Hope it is helpful to your study and work.