EN 48-2005 pdf download.Wood preservatives – Determination of eradicant action against larvae of Anobium punctatum (De Geer) (laboratory method).
5.1.1 General
Anobium punctatum (De Geer) larvae.
5.1.2 Source of larvae
Obtain the larvae from cultures reared as described in Annex D.
Cut up this wood and extract the larvae in an area separate from the test environments (5.3.1 to 5.3.3) so as to avoid the risk of introducing mites.
Prepare the storage blocks from Scots pine sapwood of dimensions (50 x 25 x 15) mm, each with 10 evenly spaced holes (see 8.1)dnlled Into one of the wide longitudinal faces with the drill (5.3.4).
Before inserting the larvae into the storage blocks, keep them overnight in small glass receptacles.
Then sort the larvae into small, medium and large sizes.
Do not use the large larvae, with a mass greater than 5 mg, for this test1).
The larvae shall be distributed as evenly as possible according to their mass. For example, for a single test the 216 larvae shall be distributed in 18 groups each of 12 larvae the mean mass of the larvae in each group shall be approximately 3,5 mg2).
Examine all the other larvae under a binocular microscope and destroy those which are damaged or infested with mites, keeping only those that are in perfect condition.
Insert the small and medium larvae into separate sets of storage blocks, placing each larva head first into a drilled hole.
Keep these filled storage blocks, holes uppermost, in glass containers covered with filter paper fixed with adhesive. Keep the larvae in the storage blocks in the culturing chamber (5.3.1) for not less than 2 months before using them in a test.
5.1.3 Provision of larvae
Carefully split the storage blocks, extract the larvae and examine them under a binocular microscope. Destroy any larvae that show injury or mite infestation. or that do not respond by movement when touched. It is particularly important to avoid including mite-Infested larvae (see Annex C).
Keep those larvae that are between 2 mg and 5 mg1) in mass and in perfect condition overnight, separate from
another, In clean receptacles in the culturing chamber (5.3.1).
Then re-examine them, and again reject any which do not show normal movements.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions In which water is the continuous phase.
NOTE Paraffin wax with a setting range from 52 °C to 54 C has been found to be suitable.
5.2.2 Gelatin, for sealing the relevant faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase.
5.2.3 Water, complying with grade 3 of EN ISO 3696.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ±2) °C, and at relative humidity (80 ± 5) %.
5.3.2 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials. Avoid
excessive exposure of operators to solvents or their vapours.
5.3.3 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).
5.3.4 Drill, provided with a bit capable of drilling cylindrical or conical holes as specified in 8.1.
5.3.5 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator.
5.3.6 Ordinary laboratory equipment, including two balances capable of weighing to an accuracy of 0.01 g.
5.3.7 X-ray apparatus. (optional) with tungsten target and beryllium window, with voltage and current
continuously variable in the ranges:
— voltage: 10 kV to 50 kV;
— current:OmAto 15 mA.
5.3.8 Protective gloves
6 Sampling
The sample of preservative shall be representative of the product to be tested, Samples shall be stored and handled in accordance with any written recommendations from the supplier.
NOTE For the samphng of preservatives from bulk supplies. the procedure given in EN 212 should be used,
7 Test specimens
7.1 Species of wood
The reference species are:
— Scots pine (Pinus syivestris Linnaeus);
— beech (Fagus syivatica Linriaeus).
NOTE Additional tests may be camed out using other species but if so. this should be stated in the test report.
7.2 Quality of wood
Use only sound wood, straight-grained without knots. For Scots pine only, sapwood with a low resin content shall be used and for beech, wood free from ‘red-heart’.
Cut the test specimens from wood of average growth rate (2,5 to 8 annual rings per 10 mm for pine, 2 to 6 annual rings per 10 mm for beech).
The proportion of summer wood in the annual rings shall not exceed 30 % of the whole in the case of pine.
The wood shall neither have been floated nor subjected to any chemical or heat treatment3). It shall be air dried and shall not have been stored for more than 5 years.EN 48-2005 pdf download.