BS EN 14103:2020,Fat and oil derivatives – Fatty Acid Methyl Esters (FAME) – Determination of ester and linolenic acid methyl ester contents.BS EN 14103:2020 pdf free download.
The purpose of BS EN 14103:2020 is to describe a procedure for the determination of the ester content in fatty acid methyl esters (FAME) intended for incorporation into diesel oil. It also allows determining the linolenic acid methyl ester content. It allows verifying that the ester content of FAME is greater than 90 % (m/m) and that the linolenic acid methyl ester content is between 1 % (m/m) and 15 % (m/m).
The precision was established using FAMEs with an ester content of 95 % (m/m) and 100 %(m/m) only, thus covering the range of the limit value. The method is also suitable outside of this range however, precision for lower concentrations is subject to further work.
This method is suitable for FAME which contains methyl esters between C6 and C24.
This method was elaborated for FAME samples from usual raw material. For FAME sample from identified raw material, a solution of the test sample is prepared without any internal standard addition, in order to verify the absence of natural nonadecanoic acid methyl ester or other unknown substances co-eluting with the IS.
NOTE 2 The calculation method of the pattern of fatty acid methyl esters is given in Anne C.
4 Principle
Determination of the mass percentage of total methyl esters of fatty acids and the mass percentage of linolenic acid methyl ester present in the sample, by gas chromatography according to a procedure using internal calibration (nonadecanoic acid methyl ester).