BS EN ISO 18363-2:2018,Animal and vegetable fats and oils – Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS – Part2:Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol.
The ISO 18363 series is a family of International Standards which can be used for the determination of ester-bound MCPD and glycidol. This introduction describes the methods specified in the three documents currently published or proposed so that the analyst can decide which methods are suitable for application. The detailed application of each method is contained within the scope of the individual method.
BS EN ISO 18363-2:2018 specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD)with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte.
This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
8.3 Matrix removal
Remove the organic phase by Pasteur pipette. Wash the aqueous phase in each assay with a second600μ of iso hexane.
8.4 Derivatization
Using new Pasteur pipettes, extract each essay three times with 600 ul of 3: 2 (v: V) mixture of diethyl ether/ethyl acetate. Take care that the pipette does not touch the aqueous phase For each assay A and B, combine the organic extracts in a new screw cap vial containing a small amount of anhydrous sodium sulfate.