BS EN ISO 20743:2021 pdf download.Textiles一Determination of antibacterial activity of textile products.
This document specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.
This document is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after- treatment or grafting).
This document covers three inoculation methods for the determination of antibacterial activity:
a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);
b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);
c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).
NOTE Based on the intended application and on the environment in which the textile product is to he used, and also on the surface properties of the textile properties, the user can select the most suitable inoculation method.
This document also specifies the colony plate count method and the adenosine tripliosphate (ATP) luminescence method for measuring the enumeration of bacteria.
2 Normative references
The töllowing documents are reterred to in the text in such a way that some or all ol their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 6330. Textiles — Domestic washing and drying procedures for textile testing
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.isu.org/ohp
— IEC Electropedia: available at http://www.clectropedia.org/
4 Safety precaution
The test methods specified in this document require the use of bacteria.
These tests should be carried out by persons with training and experience in the use of microbiological techniques.
Appropriate safety precautions should be observed with due consideration given to country-specific regulations.
5 Apparatus
Usual laboratory apparatus and, in particular, the following.
5.1 Spectrophotometer, capable of measuring at a 620 nm to 660 nm wavelength, or McFarland’s nephelometer.
5.2 Incubator, capable of maintaining a constant temperature of 37 °C ± 2 °C.
5.3 Water baths, one capable of maintaining a constant temperature of 46 °C ± 2 °C and another
capable of maintaining a temperature of 70 °C to 90 °C.
5.4 Mixer, producing a vortex shaking action.
5.5 Stomacher, capable of speeds of 6 blows per second to 8 blows per second, with the corresponding
disposable containers.
5.6 Clean bench, for microbial test.
5.7 Washing machine, in accordance with the specifications of ISO 6330.
5.8 HumIdity chamber, tropical chamber or other container capable of maintaining a high-humidity
more than 70 % RH atmospheric condition.
5.9 Luminescence photometer, capable of measuring ATP of 10-12 mol/l to 10-7 mol/l at 300 nm to
650 nm with a luminescence-measuring reagent.
5.10 Printing apparatus, capable of applying a 4 N load to a test specimen and rotating the specimen
180° in one direction for a period of 3,0 s.
5.11 Refrigerator, capable of maintaining a temperature of between 2 °C and 8 °C.
5.12 Freezers, one adjustable to a temperature below —70°C and another to a temperature below —20°C.
5.13 Balance, with the resolution of 0,01 g or better and Oil mg or better
5.14 Filtering apparatus, consisting of an upper container equipped with a membrane filter and a lower container equipped with a suction opening.
5.15 Pipette, having the most suitable volume for each use, with a tip made of glass or plastic, and with a tolerance of 0,5 % or less.
5.16 Vials, 30 ml glass bottles, with screw openings, polytetrafluoroethylene or silicone packing and caps made of polypropylene, polycarbonate or another suitable material.
5.17 Petri dishes, that have been sterilized, made of glass or plastic, in diameter sizes of 90 mm to
100 mm or 55 mm to 60 mm.
5.18 Glass rod, with a diameter of approximately 18 mm.
5.19 Anti-bumping granules (glass beads), with a diameter of 3 mm to 4 mm.
5.20 Erlenmeyer flask, of capacity 100 ml.
5.21 Cutting template, made of a sterilizable material (stainless steel or glass) one with a diameter of
38 mm ± 1 mm and the other with the diameter 60 mm ± 1 mm.BS EN ISO 20743 pdf free download.