ASTM E3151-18 pdf free download.Standard Test Method for Determining Antimicrobial Activity and Biofilm Resistance Properties of Tube, Yarn, or Fiber Specimens.
This test method is designed as an in vitro, quantitative assay to evaluate the antimicrobial activity of specimens with tubular geometries or small segments of yarn or fibers that have been treated with an antimicrobial agent. Further, the method was designed to provide a quantitative assessment of a specimen’s ability to resist microbial colonization and subsequent biofilm formation relative to an untreated control specimen.
Referenced Documents
ASTM E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods
ASTM E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
ASTM E2756 Terminology Relating to Antimicrobial and Antiviral Agents
The control and treated test specimens are placed into individual 6 × 50 mm culture tubes containing suspensions of a known biofilm-producing strain of Staphylococcus epidermidis (ATCC 359843) at a specific titer and incubated at 35 ± 2 °C with mild agitation for 24 ± 2 h.
After the contact time, each specimen is transferred to an individual centrifuge tube containing a sterile buffered saline rinse solution, and the tube is sealed and carefully inverted several times to remove any non-adhered or loosely adhered bacteria.
Although a number of standardized tests currently exist for assessing the antimicrobial activity of treated polymers and textiles, these are optimized for specimens that readily absorb the test inoculum or that have a flat surface on which the inoculum can be placed, and their use for specimens with tubular geometries or for small quantities (less than 0.5 g) of yarns or fibers requires significant manipulation of the specimen.
Reagent grade chemicals shall be used in all tests. Unless otherwise indicated, it is intended that all reagents shall conform to the specifications of the committee on Analytical Reagents of the American Chemical Society, where such specifications are available.4 Other grades may be used, provided it is pure enough to be used without lessening the accuracy of the determination.